The Vienna Drosophila RNAi Center (VDRC, website http://www.vdrc.at) was established in April 2007 as non-profit research infrastructure by the IMP and IMBA at the Campus Vienna Biocenter. Its mandate is to maintain and distribute the transgenic RNAi stocks constructed by Dickson and Keleman groups at the IMP. From March 1, 2011, the VDRC formally became independent of IMP and IMBA and joined the Campus Science Support Facilities (CSF). With this move the VDRC became part of Austria’s first long-term funding for research infrastructure. Currently approximately half of the VDRC operating costs are covered by user fees, the other half by funding of the Austrian Federal Government (bm.wf) and the City of Vienna.
The Vienna Drosophila RNAi Center aims to promote scientific discoveries by facilitating systematic analysis of gene function in Drosophila using in vivo transgenic technology. Our immediate goals are to maintain and distribute transgenic RNAi lines and other resources to the Drosophila researchers, both locally and world-wide, and in future, to further develop and expand VDRC resources according to the emerging new technologies and community needs.
1. Genome-wide RNAi libraries (GD and KK)
Currently the VDRC maintains and makes available a total of 31,920 Drosophila transgenic RNAi lines, consisting of:
Collectively, the GD and KK collections cover a total 13,264 Drosophila genes (93.8%), with GD collection covering 11,972 genes (84.6%) and KK collection covering 9502 genes (67.2%). For most genes, more than one independent RNAi line is available through the VDRC.
In addition, the VDRC provides 13,848 DNA constructs used to generate the GD collection and 16 miscellaneous stocks used for construction of both RNAi collections.
2. GAL4- Driver library (VT - Vienna Tiles)
This library consists of transgenic Drosophila lines containing short fragments of genomic DNA controlling GAL4 expression. The constructs are integrated at a single defined genomic position and each carries a distinct ~2kb long candidate cis-regulatory DNA fragment controlling GAL4 expression. The short fragment size was deliberately chosen to enable expression of exogenous genes in a limited number of cells.
The precise expression patterns of reporters driven by these lines have been extensively documented and annotated, both in the Drosophila adult brain (Dickson group, BrainBase) and spatio-temporally in embryos (unpublished data, Stark group, www.starklab.org)
Due to their sparse expression patterns, the VT lines will dramatically facilitate the study and manipulation of gene activity in reproducibly small, distinct and defined subsets of cells.
VDRC Delivery Statistics
To date, the VDRC has delivered more than 800,000 RNAi lines to almost 1.850 registered users all over the world.
VDRC is staffed with 21 employees:
Maintenance and distribution supervisor
Software and database developer
Administrative assistant (part time)
Stocks maintenance and shipping technicians
Head of the VDRC: Lisa Meadows
Scientific Advisory Board (SAB):
Campus User Committee (VCUC):