UAS-RNAi lines are a powerful tool for facilitating rapid loss-of-function experiments. When crossed with a GAL4 driver line, our genome-wide tissue-specific Drosophila RNAi resource enables large scale genetic screens, making it possible to study gene functions in essentially any tissue or cell at any stage in the life of the organism.
Currently the VDRC maintains and makes available a total of 26,585 Drosophila transgenic RNAi lines, developed and created by Barry Dickson and Krystyna Keleman, IMP/IMBA, Vienna, Austria, consisting of:
- GD library, 16,763 lines. P-element based transgenes, random insertion sites. Available since April 2007. See here to browse the GD RNAi stock collection.
- KK library, 9,822 lines. phiC31-based transgenes, single, defined insertion site. Available since April 2009. See here to browse the KK RNAi stock collection.
Collectively, the GD and KK collections cover a total 12,671 Drosophila protein-coding genes (91%), with GD collection covering 11,292 genes (81%) and KK collection covering 9,646 genes (69%). For most genes, more than one independent RNAi line is available through the VDRC.
In addition, the VDRC provides :
- 13,848 DNA constructs used to generate the GD collection
- and 16 toolkit stocks used for construction of both RNAi collections.
See here for further details about the RNAi libraries
GAL4 lines are one part of the binary GAL4-UAS system (Brand and Perrimon, Development 1993). GAL4 'drivers' are typically used in flies to restrict the expression of a UAS line (eg. UAS-RNAi) to a specific subset of cells or developmental time-point.
VDRC maintains and distributes a collection of 8,457 VT (Vienna Tile) GAL4 driver lines, generated and validated by the Dickson and Stark groups at the IMP, Vienna. Each line contains a short fragment of genomic DNA controlling GAL4 expression and the constructs are integrated at a single defined genomic position (attP2).
The precise expression patterns of reporters driven by these lines have been extensively documented and annotated. Data from these analyses are stored in the following databases and are invaluable for helping you search for lines of interest and are directly linked to the VDRC stock ID for ease of ordering:
- Adult brain http://brainbase.imp.ac.at/bbweb
Primary user interface to efficiently browse and retrieve confocal microscopy data and related annotated anatomical structures.
- Embryos http://enhancers.starklab.org
Enables simple and complex searches by IDs (VT tile number), neighboring genes, genomic coordinates and annotation terms including embryonic developmental stage, strength of enhancer activity, fixed anatomical terms and keywords.
See further information about VT GAL4 lines.
Browse the VT lines.
See associated publication.