Vienna Drosophila Resource Center

  • Drosophila resources for researchers worldwide.
  • Drosophila resources for researchers worldwide.
  • Drosophila resources for researchers worldwide.
  • Drosophila resources for researchers worldwide.
  • Drosophila resources for researchers worldwide.

The Vienna Drosophila Resource Center (VDRC) is a professionally organized bioresource center which aims to promote scientific discoveries in Drosophila, primarily by maintaining transgenic Drosophila melanogaster stocks and DNA resources and distributing them to Drosophila researchers both locally and worldwide.

In future, we plan to develop and expand VDRC resources further according to the emerging new technologies and community needs.

With nearly 90% coverage of the protein-coding genes, VDRC maintains and distributes the largest collection of Drosophila RNAi lines worldwide, enabling almost genome-wide screens.

VDRC maintains and distributes a collection of 8,500 Vienna Tiles (VT) Gal4 driver lines whose expression patterns have been characterized throughout embryonic development and in the adult brain.

Services

    Fly stocks distribution

    Fly stocks distribution

    VDRC maintains more than 38,000 independent transgenic fly lines, nearly all in duplicate. We are able to ship to any Drosophila researcher who has obtained a VDRC log-in and, as of 2014, have shipped over 1 million lines worldwide.

    The stocks currently maintained are primarily RNAi lines, but we also have a substantial collection of enhancer-GAL4 driver lines plus others which have been deposited by research groups outside of VDRC.

    For more information and stock ordering go to www.vdrc.at.

    Plasmid DNA distribution

    Plasmid DNA distribution

    In addition to maintaining transgenic fly stocks, VDRC is also a repository for plasmid DNAs which are of use to Drosophila researchers. We are able to ship plasmids to any Drosophila researcher who has obtained a VDRC log-in.

    We currently make available 13,840 DNA constructs used to generate the GD RNAi transgenic fly collection. These are long hairpin constructs for a specific gene inserted in the pMF3 vector (see GD library cloning strategy).

    In addition we have a small collection of plasmids useful for Drosophila research which have been deposited by research groups outside of VDRC.

    Private stock keeping

    Private stock keeping

    VDRC is open to donations of highly used Drosophila stocks for integration into its community stock center collection. In addition, we offer a private stock keeping service where we will maintain and distribute your personal fly stock/plasmid collection on your behalf. See [here] for information about the VDRC Stock Keeping Policy.

    If you wish to donate resources to VDRC or use our private stock keeping service, please contact us to discuss as early as possible: office@@vdrc.at.

    Generation of new transgenic lines

    Generation of new transgenic lines

    Service coming soon...

    Resources

    RNAi libraries

    UAS-RNAi lines are a powerful tool for facilitating rapid loss-of-function experiments. When crossed with a GAL4 driver line, our genome-wide tissue-specific Drosophila RNAi resource enables large scale genetic screens, making it possible to study gene functions in essentially any tissue or cell at any stage in the life of the organism.

    Currently the VDRC maintains and makes available a total of 26,585 Drosophila transgenic RNAi lines, developed and created by Barry Dickson and Krystyna Keleman, IMP/IMBA, Vienna, Austria, consisting of:

    • GD library, 16,763 lines. P-element based transgenes, random insertion sites. Available since April 2007. See here to browse the GD RNAi stock collection.
    • KK library, 9,822 lines. phiC31-based transgenes, single, defined insertion site. Available since April 2009. See here to browse the KK RNAi stock collection.

    Collectively, the GD and KK collections cover a total 12,671 Drosophila protein-coding genes (91%), with GD collection covering 11,292 genes (81%) and KK collection covering 9,646 genes (69%). For most genes, more than one independent RNAi line is available through the VDRC.

    In addition, the VDRC provides :

    • 13,848 DNA constructs used to generate the GD collection
    • and 16 toolkit stocks used for construction of both RNAi collections.

    See here for further details about the RNAi libraries

    Enhancer-GAL4 driver library

    GAL4 lines are one part of the binary GAL4-UAS system (Brand and Perrimon, Development 1993). GAL4 'drivers' are typically used in flies to restrict the expression of a UAS line (eg. UAS-RNAi) to a specific subset of cells or developmental time-point.

    VDRC maintains and distributes a collection of 8,457 VT (Vienna Tile) GAL4 driver lines, generated and validated by the Dickson and Stark groups at the IMP, Vienna. Each line contains a short fragment of genomic DNA controlling GAL4 expression and the constructs are integrated at a single defined genomic position (attP2).

    The precise expression patterns of reporters driven by these lines have been extensively documented and annotated. Data from these analyses are stored in the following databases and are invaluable for helping you search for lines of interest and are directly linked to the VDRC stock ID for ease of ordering:

    • Adult brain http://brainbase.imp.ac.at/bbweb
      Primary user interface to efficiently browse and retrieve confocal microscopy data and related annotated anatomical structures.
    • Embryos http://enhancers.starklab.org
      Enables simple and complex searches by IDs (VT tile number), neighboring genes, genomic coordinates and annotation terms including embryonic developmental stage, strength of enhancer activity, fixed anatomical terms and keywords.

    See further information about VT GAL4 lines.
    Browse the VT lines.

    See associated publication.

     

     

    Plasmids

    In addition to maintaining and distributing transgenic fly lines, VDRC also acts as repository for plasmids which are useful for Drosophila research.

    Currently we make available 13,840 DNA constructs used to generate the GD RNAi transgenic fly collection (see DNA construct list). These are long hairpin constructs for a specific gene inserted in the pMF3 vector (see GD library cloning strategy).

    In addition we have a small collection of plasmids useful for Drosophila research which have been deposited by research groups outside of VDRC and we aim to add to this collection. Plasmids in this category, such as bicistronic CRIPSR/Cas9 plasmids used for rapid and versatile genome editing, can be found under Other Resources.

    Other resources

    In addition to maintaining and distributing transgenic fly stocks and plasmids owned by the VDRC, we also maintain Drosophila resources owned by other organizations and distribute them to the scientific community on behalf of the depositor ('Provider Scientist') via the regular VDRC webshop. For more information see About Other Resources.

    The first such resources available are bi-cistronic CRISPR/Cas9 vectors deposited by Peter Duchek from IMBA, Vienna (see here).

    Further fly stocks and plasmids will be added to the 'Other Resources' section as soon as the 'Provider Scientist' wishes to make them available to researchers with a VDRC login.

    User Information

    How to order resources

    • Log into VDRC website using your user name & password. If you do not have a user name yet please register first.
    • You can search for the stocks / DNA constructs for the gene of interest by entering CG number, synonym or Transformant ID (using 'Quick Search'). For more detailed search please use the 'Advanced Stock Search Form'. If you want to order large number of stocks / DNA constructs you may find convenient to use ’Search File Upload’.
    • Submit your order and check out by providing your credit card information.
    • You will receive an email confirmation with the invoice attached in PDF format.
    • You can review all your ordered stocks / DNA constructs in your Order History.

    Policy

    VDRC Terms and Conditions

    • RNAi lines, other stocks and DNA constructs are to be used only under conditions specified in the ATA.
    • User fees are payable in advance only by charge to VISA or MC account.
    • Due to the higher price charged, orders of 100 items or less will be dispatched within 2 weeks from the ordering date. Orders above 100 items will be dispatched within 6 weeks. If any item cannot be dispatched the researcher will be fully refunded for that part of the order to their VISA or MC account.
    • Orders charged at 'fast' dispatch service rate will be dispatched within 2 weeks of receipt of order. If VDRC fails to dispatch your order in time you will be refunded 50% of the total cost of the order and your order will be dispatched as soon as possible thereafter.
    • Order cancellation with full refund is possible within 24hrs from the ordering date. Please contact office@@vdrc.at.
    • Invoice will be sent by e-mail and signed digitally.
    • The VDRC does not guarantee that any line is effective, nor that it contains the intended transgene/genetic modification.
    • Shipping is only possible by FedEx or DHL. Delivery terms are ex works (Incoterms 2000). The recipient pays all transportation costs, applicable taxes and applicable customs duties.
    • The VDRC takes no responsibility for the condition of the sent flies due to the shipment.
    • If the order is ready to ship, but no valid permit is present, you will be informed via email. If we do not receive all the necessary documents within two weeks, the VDRC has the right to discard the order and only pending lines will be refunded.
    • If the package is refused or discarded at the customs (or at an import regulation agency) due to invalid import permit, VDRC will not replace the lines or the shipping costs.

    Links

    Citing the Facility

    Continuation of funding for VDRC by the City of Vienna and the Austrian Ministry of Science is dependent on documented evidence of contributions to scientific output.

    Therefore it is absolutely required to acknowledge use of the facility when publishing work in which VDRC services were utilized.

    A simple statement is sufficient and can either be placed in the Materials and Methods section or in the Acknowledgements.

    Suggested format:
    Transgenic fly stocks and/or plasmids were obtained from the Vienna Drosophila Resource Center (VDRC, www.vdrc.at).
    Your publication will then be listed on our website.

    Citing RNAi lines:
    For RNAi lines, please cite Dietzl et al [Nature 448,151-156,12 Jul 2007] and acknowledge the VDRC.

    Publications

    Genome-scale functional characterization of Drosophila developmental enhancers in vivo.
    Kvon EZ, Kazmar T, Stampfel G, Yáñez-Cuna JO, Pagani M, Schernhuber K, Dickson BJ, Stark A.
    Nature. 2014 Aug 7;512(7512):91-5 (Abstract)

    Abdominal-B neurons control Drosophila virgin female receptivity.
    Bussell JJ, Yapici N, Zhang SX, Dickson BJ, Vosshall LB.
    Curr Biol. 2014 Jul 21;24(14):1584-95 (Abstract)

    A transcriptome-wide RNAi screen in the Drosophila ovary reveals factors of the germline piRNA pathway.
    Czech B, Preall JB, McGinn J, Hannon GJ.
    Mol Cell. 2013 Jun 6;50(5):749-61. (Abstract)

    The genetic makeup of the Drosophila piRNA pathway.
    Handler D, Meixner K, Pizka M, Lauss K, Schmied C, Gruber FS, Brennecke.
    J.Mol Cell. 2013 Jun 6;50(5):762-77. (Abstract)

    Team

    Lisa Meadows

    Lisa Meadows

    Core Facility Head
    VBC2 / PG6

    Reinhard Klug

    Reinhard Klug

    Maintenance and Distribution Supervisor
    VBC2 / PG6

    Lydia Gaillard

    Lydia Gaillard

    Administration
    VBC2 / PG6

    Yulia Barinova

    Yulia Barinova

    Technician
    VBC2 / PG14

    Kristina Belogradova

    Kristina Belogradova

    Technician
    VBC2 / PG14.1

    Tamara Burger

    Tamara Burger

    Technician
    VBC2 / PG14.1

    Lucien Chesnay

    Lucien Chesnay

    Technician
    VBC2 / PG14

    Michaela Eckmann-Mader

    Michaela Eckmann-Mader

    Technician
    VBC2 / PG14.1

    Randolph Egleston

    Randolph Egleston

    Technician
    VBC2 / PG14.1

    Natalia Hallay

    Natalia Hallay

    Technician
    VBC2 / PG14.1

    Rainer Keck

    Rainer Keck

    Technician
    VBC2 / PG14.1

    Joanna Morrobel-Peret

    Joanna Morrobel-Peret

    Technician
    VBC2 / PG14.1

    Barbara Muellner

    Barbara Muellner

    Technician
    VBC2 / PG14.1

    Irene Penzenauer

    Irene Penzenauer

    Technician
    VBC2 / PG14

    Elena Popowich

    Elena Popowich

    Technician
    VBC2 / PG14

    Barbara Ruplitsch

    Barbara Ruplitsch

    Technician
    VBC2 / PG15

    Jadwiga Rybarczyk

    Jadwiga Rybarczyk

    Technician
    VBC2 / PG14.1

    Alisher Tashpulatov

    Alisher Tashpulatov

    Technician
    VBC2 / PG14

    Sandor Urmosi-Incze

    Sandor Urmosi-Incze

    Technician
    VBC2 / PG14.1

    Judith Utner

    Judith Utner

    Technician
    VBC2 / PG15

    Svetlana Zorinyants

    Svetlana Zorinyants

    Technician
    VBC2 / PG14.1

    Contact

    Expression data

    Data from these analyses are stored in the following databases and are invaluable for helping you search for lines of interest and are directly linked to the VDRC stock ID for ease of ordering.

    Adult brain

    http://brainbase.imp.ac.at/bbweb
    Primary user interface to efficiently browse and retrieve confocal microscopy data and related annotated anatomical structures. (Developed by Biomedical Visualization Group at the Research Center for Virtual Reality and Visualization VrVis, Vienna and Dickson group, IMP, Vienna (now at Janelia Farm).

    Embryos

    http://enhancers.starklab.org
    This site enables manual inspection of the raw microscopy slides at arbitrary zoom levels and allows simple and complex searches by:

    • IDs (VT tile number)
    • neighboring genes (e.g. FBgn, synonyms)
    • genomic coordinates
    • annotation terms including:
      • embryonic developmental stage
      • strength of enhancer activity
      • fixed anatomical terms
      • keywords

    See here for further information about VT GAL4 lines.
    See here to browse the VT lines.